Zebrafish earns its stripes for in vivo ASC speck dynamics

Zebrafish earns its stripes for in vivo ASC speck dynamics

Assembly of the ASC speck is critical for signaling by the inflammasome. In this issue, Kuri et al. (2017. J. Cell Biol. https://doi.org/10.1083/jcb.201703103) use live microscopy to track fluorescently tagged endogenous ASC in the zebrafish, describing the molecular domains driving ASC speck assembly and identifying a key role for macrophages in ASC speck removal in vivo.

Dynamics of in vivo ASC speck formation

Activated danger or pathogen sensors trigger assembly of the inflammasome adaptor ASC into specks, large signaling platforms considered hallmarks of inflammasome activation. Because a lack of in vivo tools has prevented the study of endogenous ASC dynamics, we generated a live ASC reporter through CRISPR/Cas9 tagging of the endogenous gene in zebrafish. We see strong ASC expression in the skin ...

Correction: Dynamics of in vivo ASC speck formation

Dynamics of ASC speck formation during skin inflammatory responses in vivo

17 Activated danger or pathogen sensors trigger assembly of the inflammasome adaptor ASC into 18 specks, large signalling platforms considered hallmarks of inflammasome activation. Because a 19 lack of in vivo tools has prevented the study of endogenous ASC dynamics, we generated a live 20 ASC reporter through CRISPR/Cas9 tagging of the endogenous gene in zebrafish. We see strong 21 ASC express...

How the zebrafish got its stripes

H ow the zebra got its stripes is a frequent topic in popular literature about the natural world. For developmental biologists, however, it is much easier to study the biology of pattern formation in zebrafish, where the tools of experimental embryology and genetics have proved to be very useful. Now, in eLife, David Parichy from the University of Washington and coworkers – including Dae Seok E...